Methods the 54th generation of transformed human embryonic tendon cells and artificial composite materials of carbon fibers ( cf ) and polyglycolic ( pga ) were co - cultured in vitro to construct tet . lt was frozen in liquid nitrogen with four kinds of cpa for 2 months . post - thawed quickly and transplanted into hind limbs of nude mice , and repaired the defects of achilles tendon . after 2 , 4 , 6 , 8 , 12 weeks , the morphological , histological , ultrastructure , short tandem repeat loci and immunohistochemistry examination were detected , and biomechanical strength of tet were examined . result tendon cell survived and could secret type i collagen after 12 weeks to transplanted into nude mice . in the group of dmso + raffmose + kh2o4 , vacuole in mitochondrion degraded i tendon cell ranged in order , abundant collagen fibers were found and linked each other and the biomechanical strength was increased as time elapsed . c onclusion dmso + raffmose + kh2o4 could protect tet in deep low temperature 组织工程肌腱制备完成后在四种抗冻剂保护下液氮冻存2月；快速复温后植入裸鼠以修复跟腱缺损， 2 、 4 、 6 、 8 、 12周后取出，观察形态学、组织学、电镜和免疫组织化学变化，短串联重复位点检测和生物力学变化。结果实验组组织工程肌腱体内植入12周后仍有肌腱细胞存活并分泌型胶原；随着时间延长, 10二甲基亚砜( dmso ) +棉子糖( 30mmol l ) + kh _ 2po _ 4 ( 25mmol l )组线粒体空泡减少，肌腱细胞排列整齐，胶原纤维增粗并连接，抗拉强度增高。
